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. 2013 Dec;19(12):1659–1668. doi: 10.1261/rna.039388.113

FIGURE 2.

FIGURE 2.

Growth rate and RNA analyses of mpp6Δ and rex1Δ mutants upon Rrp6NT induction. (A) Growth rate analyses of wild-type, rrp47Δ, mpp6Δ, and rex1Δ strains after induction of GST or GST-Rrp6NT. Cultures were grown in galactose-based minimal medium at 30°C and maintained in early exponential growth by dilution with pre-warmed medium. The increase in OD600 is expressed as log2 values. All plotted lines of best fit have Rf values greater than 0.98. (B) Lanes 1–8, acrylamide gel Northern analyses of RNA from wild-type, rrp47Δ, mpp6Δ, or rex1Δ strains expressing either GST (−) or GST-Rrp6NT (+). RNA was isolated from cultures grown in selective galatose-based medium. Lanes 9–12, Northern analyses of RNA from a rex1Δ mutant and from a rex1Δ rrp47Δ mutant expressing either the full-length RRP47 gene, the rrp47ΔC allele, or the rrp47I162X allele. RNA was isolated from cultures grown in glucose-based selective minimal medium. The blot was successively hybridized with probes complementary to the RNA species indicated. Identical hybridizations and exposure times are shown for lanes 1–8 and 9–12.