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. 2013 Dec;19(12):1755–1766. doi: 10.1261/rna.040204.113

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© 2013 Rage et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 1. — Identification of SMN-associated RNAs. (A) Flow chart showing the sequential steps used for identification of SMN-associated RNAs at genome-wide scale. (B) Western blot analysis of endogenous (Endo-SMN) and exogenous (Flag-SMN) SMN expression levels in noninduced (NI) and induced (I) 3xFlag-SMN NSC-34 cells. (C) Western blot analysis of SMN-interacting hnRNP Q/R proteins in total extracts and immunoprecipitates from NSC-34 cells expressing 3xFlag-SMN (SMN) or no 3xFlag construct (Ctl). Total corresponds to ∼1% of the input used for IP. (D) RT-PCR analysis of β-actin (Actb) and Cpg15 (Nrn1) mRNAs purified from IP samples.