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. 2013 Dec;19(12):1825–1832. doi: 10.1261/rna.040865.113

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© 2013 Cox et al. Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 4. — Argonaute (Ago) 2–dependent siRNA activities of precursor miR-122 molecules in wild-type mouse embryo fibroblasts (MEFs) and Ago2^−/− MEFs. (A) GFP-based siRNA assays in the presence of pre-miR-122 molecules, as indicated. Experiments were done as described in Figure 2D. Quantitation is shown as the ratio of full-length GFP mRNA to total (full-length plus cleaved) GFP mRNA and represents at least three independent replicates. Error bars, SEM. (B) Rescue of siRNA activities by transfection of plasmids encoding chimeric Flag-Ago2 proteins. Experiments were performed as in A but with the addition of plasmids encoding either Flag-tagged Ago2 or Flag-tagged BAP proteins. Quantitation is shown as the ratio of full-length GFP mRNA to total (full-length plus cleaved) GFP mRNA and represents at least three independent replicates. Error bars, SEM.