Table 1. Primers for RT-PCR and nested PCR testing for severe fever with thrombocytopenia syndrome virus, Shandong Province, China, 2011*.
Type of primer | Primer name | Sequence, 5′→3′ | Virus segment |
---|---|---|---|
Primary |
F1S | CAGCCACTTTACCCGAACAT | Small |
R1S |
GGAAAGACGCAAAGGAGTGA |
||
Nested |
F2S | CTGGTCTCTGCCCTCTCAAC |
|
R2S |
GGATTGCAGTGGAGTTTGGTG |
||
Primary |
F1L | GGCAGCAAACCAGAAGAAAG | Large |
R1L |
CATTTCTCCGAGGGCATTTA |
||
Nested | F2L | GGGTCTCCTGCTTAGCACAGG | |
R2L | TCAGAFAAFACCCTGCCAGT |
*RT-PCR, reverse transcription PCR; F, forward; R, reverse.