Table 2. Bacteriological and biochemical features of Brucella strains.
| Strain | TZ (twin boy) | TS (twin girl) | ML (mother) |
|---|---|---|---|
| CO2 requirement | – | – | – |
| H2S production |
– |
– |
– |
| Dye inhibition* | |||
| Thionin | + | + | + |
| Basic fuchsin |
+ |
+ |
+ |
| Mono-specific anti-serum agglutination† | |||
| A | + | + | + |
| M | + | + | + |
| R |
– |
– |
– |
| Lysis test by Brucella spp. phage‡ | |||
| Tb104 | – | – | – |
| Tb | – | – | – |
| Wb | ± | ± | ± |
| BK2 |
+ |
+ |
+ |
| Identification | |||
| Species | B. melitensis | B. melitensis | B. melitensis |
| Biovar | 3 | 3 | 3 |
*A final concentration of 20 μg/mL dyes was used in the testing (4). †The bacterial isolate was tested for agglutination by mono-specific anti-serum samples to Brucella antigens A, M, and R (rough), respectively. ‡Bacterial isolate was tested for lysis by specific Brucella phages of Tb, Wb, and BK2.