Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan;20(1):88–97. doi: 10.3201/eid2001.130858

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.

PMC Copyright notice

PMCA of PRNP codon 129MM human brain homogenate and humanized transgenic mice brain homogenate seeded with C-BSE, scrapie, CWD, L-BSE, H-BSE, vCJD, and atypical scrapie. PMCA reactions using PRNP 129MM human brain homogenate (human postmortem tissue) (A) and PRNP 129MM humanized transgenic mouse brain homogenate (Tg-Hu) (B) were seeded (1:3) with animal prion disease brain as indicated. Lanes 1, 3, 5, 7, 9, 11, 13, and 15 show the samples without PMCA. Samples in lanes 2, 4, 6, 8, 10, 12, and 14 were subjected to PMCA. Western blotting used the antibody 3F4 that enables the specific detection of human PrP. To compare the PrP^res levels into the seeds (before the PMCA), antibody 6H4 was also used. PMCA, protein misfolding cyclic amplification; BSE, bovine spongiform encephalopathy; CWD, chronic wasting disease; vCJD, variant Creutzfeldt-Jakob disease; PrP, prion protein; PrP^res, protease-resistant PrP; M, molecular marker.