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Effect of pH on absorption (Abs.) at 388 nm (a) or 498 nm (b) and fluorescence emission (Em.) at 456 nm (388 nm excitation, Ex.) (c), 507 nm (388 nm excitation) (d), and 507 nm (450 nm excitation) (e) of wild-type CoGFP and of five variant CoGFPs (mean ± SD, N = 3).
