Table 1.
A list of PCR primers used in this study. The symbols of H and N denote mixture of base, A, C and G, and A, C, G and T, respectively
| No. | Primer name | Sequence |
|---|---|---|
| 1 | COGFP-TTT | 5'-ATHCCNGATTATTTTGT-3' |
| 2 | COGFP-TTC | 5'-ATHCCNGATTATTTCGT-3' |
| 3 | COGFP-TCT | 5'-ATHCCNGATTACTTTGT-3' |
| 4 | COGFP-TCC | 5'-ATHCCNGATTACTTCGT-3' |
| 5 | COGFP-CTT | 5'-ATHCCNGACTATTTTGT-3' |
| 6 | COGFP-CTC | 5'-ATHCCNGACTATTTCGT-3' |
| 7 | COGFP-CCT | 5'-ATHCCNGACTACTTTGT-3' |
| 8 | COGFP-CCC | 5'-ATHCCNGACTACTTCGT-3' |
| 9 | COGFP-ATTAA | 5'-GAAGGNTTTACNTTTGAAAG-3' |
| 10 | COGFP-ACCAA | 5'-GAAGGNTTCACNTTCGAAAG-3' |
| 11 | COGFP-ATTGA | 5'-GAGGGNTTTACNTTTGAGAG-3' |
| 12 | COGFP-ACCGA | 5'-GAGGGNTTCACNTTCGAGAG-3' |
| 13 | GeneRacer3'Primer | 5'-GCTGTCAACGATACGCTACGTAACG-3' |
| 14 | GeneRacer3'Nested Primer | 5'-CGCTACGTAACGGCATGACAGTG-3' |
| 15 | COGFP-A-R1 | 5'-GCTATAGCCGTCTCATGTTGCTCGT-3' |
| 16 | COGFP-A-R2 | 5'-AGCCGTCTCATGTTGCTCGTAGTAG-3' |
| 17 | COGFP-A-R3 | 5'-ATGTTGCTCGTAGTAGTTGCCTTCCTCGAC-3' |
| 18 | GeneRacer5'Primer | 5'-CGACTGGAGCACGAGGACACTGA-3’ |
| 19 | GeneRacer5'Nested Primer | 5'-GGACACTGACATGGACTGAAGGAGTA-3’ |
| 20 | COGFP-A-Full-F3 | 5'-ATTTAGGTGGCTGCGTACAG-3' |
| 21 | COGFP-A-Full-F4 | 5'-ATTTAGGTGGCTGCGTACAGTTAACAC-3' |