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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Biomark Med. 2013 Aug;7(4):10.2217/bmm.13.44. doi: 10.2217/bmm.13.44

Figure 2. Relative quantitation of urinary miRNA is reflected by cycle threshold values.

Figure 2

Total RNA was isolated from one normal subject and was added to the RT reaction at: (A) various concentrations via serial dilutions of RNA by adding nuclease-free water; or (B) various volumes of the same RNA. Subsequently, miR-16 was quantitated from these reactions as described in the ‘Materials & methods’ section. Error bars indicate standard deviation. (C) Target (miR-16) and reference (U6) amplifications were performed, at the cDNA dilutions indicated, from a single urine sample. The change in CT value between miR-16 and U6 at each cDNA dilution (ΔCT) is shown.

CT: Cycle threshold; RT: Reverse transcription.