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. 2013 Dec 23;2013:956397. doi: 10.1155/2013/956397

Figure 4.

Figure 4

Effects of SYD and LY294002 on cell apoptosis. (a) Representative images of Hoechst 33342 staining. (b) Quantitative analysis of apoptosis rate. The cells were exposed to 6 hours of normoxic culture (normal), 2 hours of hypoxia followed by 4 hours of reoxygenation (H/R), postconditioning with two concentrations of SYD or control serum (5% or 10% each). In some experiments, 5% SYD was complemented with LY294002 (5% SYD + LY294002). Data are expressed as mean ± SEM (n = 3), ## P < 0.01 versus normal, **P < 0.01 versus 5% control serum. ΔΔ P < 0.01 versus 10% SYD, and ☆☆ P < 0.01 versus 5% SYD. Scale bar represents 50 μm.