Figure 2.

Static (a, c, e) and dynamic (b, d, f) tests performed for evaluating the bioreactor sterility maintenance. Test 1: evaluation of the culture system (Ismatec multichannel peristaltic pump, fluidic circuit and culture chamber) (a, b). Test 2: evaluation of the single culture chamber (c) with a disposable-sterile-silicone-pump tubing Infusomat Standard (BBraun) and fed in the infusion pump Infusomat fmS (d). Test 3: evaluation of the preassembled circuitry alone in static (e) and dynamic (f) condition, where an Ismatec peristaltic pump is used. As a control, a T-flask containing the same medium, used for all tests, was incubated (g).