. Author manuscript; available in PMC: 2014 Nov 6.

Published in final edited form as: J Am Chem Soc. 2013 Oct 25;135(44):10.1021/ja405459b. doi: 10.1021/ja405459b

Table 2.

Photophysical parameters of modBFP variants.

FP	Enhancement (372, 405 nm)	ν_c (Hz)	τ_bright (ms)	τ_decay (ms)	τ_{decay, 2} (ms)
modBFP	20%, 4%	16	100 (± 5)	167 (± 14)	157 (± 13)
H148D	15%, 4%	24	66 (± 5)	113 (± 16)	106 (± 15)
H148G	11%, 6%	30	57 (± 2)	222 (± 28)	206 (± 26)
H148K	37%, 15%	37	32 (± 2)	162 (± 35)	137 (± 30)
H148R	15%, 3 %	5^a
H148Y	5%, 2 %	6^a
V150A	20%, 10 %	31	40 (± 3)	164 (± 56)	H5 (± 50)
F165H	5%, 2 %	10^a
F165L	0	N/A

Larger uncertainty due to low fluorescence enhancement. Due to the poor spatial mode of the 372 nm (pulsed), on and off times were only determined for 405 nm (cw) enhancement.