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. Author manuscript; available in PMC: 2014 Dec 12.
Published in final edited form as: Cell Rep. 2013 Dec 12;5(5):10.1016/j.celrep.2013.11.012. doi: 10.1016/j.celrep.2013.11.012

Figure 1. SMN is required for U7 snRNP biogenesis.

Figure 1

(A) Western blot analysis of NIH3T3-SmnRNAi and NIH3T3-SMN/SmnRNAi cells cultured with or without Dox for 5 days. (B) In vitro U1 and U7 snRNP assembly with wild-type NIH3T3 cell extracts. (C) In vitro U1 and U7 snRNP assembly with extracts from NIH3T3-SmnRNAi and NIH3T3-SMN/SmnRNAi cells cultured with or without Dox for 5 days. (D) Quantification of U7 snRNP assembly from three independent experiments as in (C). (E) Northern blot analysis of NIH3T3-SmnRNAi and NIH3T3-SMN/SmnRNAi cells cultured with or without Dox for 5 days. (F) Quantification of U7 levels normalized to 5S rRNA from three independent experiments as in (E). (G) Schematic of the U7 gene and sequence of the U7 snRNA precursor.

Data in all graphs are represented as mean and SEM. See also Figure S1.