Fig 2.

Washing vials does not affect the degree of contaminating zinc-mediated GlyR enhancement. EC₅ glycine was determined in the presence of 2.5 mM tricine in polypropylene or glass vials and used for solutions prepared in the absence of tricine in vials that were either not washed, washed five times with diH₂O, washed 50 times with diH₂O H₂O, or submitted to a 10 min. soak in MBS + 2.5 mM tricine prior to drying and use. Data are reported as percent current of that elicited by EC₅ glycine in the presence 2.5 mM tricine. A) Summary data for currents elicited by EC₅ glycine solutions prepared in polypropylene tubes under the various wash conditions. B) Summary data for currents elicited by EC₅ glycine solutions prepared in glass vials under the various wash conditions. Data are presented as the mean ± S.E.M. of 3 – 5 oocytes.