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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Mol Cell Cardiol. 2013 Nov;64:10.1016/j.yjmcc.2013.09.005. doi: 10.1016/j.yjmcc.2013.09.005

Figure 3.

Figure 3

MSC27a cells exhibit a pericytic phenotype, and manipulation of known angiogenic pathways does not significantly affect vascular formation in engineered tissue constructs. a, Array expression data heatmap of pericyte and EC markers in two distinct MSC clones (MSC5 and MSC27a) and in corresponding engineered tissues also containing ECs (labeled with “gene name (protein)”) shows a general trend towards increased expression in EC:MSC27a tissues. Scale indicates highly expressed (red) or minimally expressed (blue) genes based on a row-level z-score calculated as (x-xmean)/StDev (where values are expressed as log2). Grey color indicates expression was not detected. Note that CD144 and CD31 were highly expressed in EC:MSC27a patches compared to EC:MSC5 patches, likely due to improved EC survival in the former engineered tissues. b, Manipulation of known angiogenic pathways VEGF and NOTCH in engineered tissues with VEGF-A165 at 10 or 100 ng/ml (10V or 100V), or with 10ng/ml VEGF-A165 and DAPT at 0.2, 2, or 20 μM (10V + 0.2D, 2D, or 20D, respectively) showed no changes in vessel structure development after 8 days in culture (n=3 per group, P=not significant by ANOVA).