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. Author manuscript; available in PMC: 2014 Nov 5.
Published in final edited form as: Anal Chem. 2013 Oct 23;85(21):10.1021/ac4026048. doi: 10.1021/ac4026048

Figure 5.

Figure 5

Analysis of biological reaction efficiency in picoliter droplets. (A) Fluorescence images of droplet RPA using the Jeffamine aqueous additive with unmodified Krytox surfactant. Leftmost images show grayscale (and composite) images taken post-RPA using the Red, Transmission (DIC), and Green imaging filters. Red droplets represent zero template controls; all other droplets contained 16 pM template (670 copies/droplet). Composite image (R + G) shows that none of the control droplets generated products. Rightmost image shows snapshots of the real-time RPA reaction; control droplet outlined in red; template-containing droplet outlined in green. (B) RPA reaction kinetics monitored in the presence of unmodified surfactant with no additive (black), Jeffamine-bound surfactant (blue), and KryJeffa surfactant (orange). Average traces from 3 representative droplets are shown with standard deviations. (C) Following droplet RPA with 0 or 16 pM template, droplet population intensities were counted with each treatment, indicating similar efficiency with KryJeffa and Jeffamine-bound surfactants. The abbreviation “cpd” refers to DNA copies per droplet.