Table 3. Primers for genes of interest and reference genes used for real-time qPCR.

Gene Symbol	Gene Name	NCBI Accession Number	Annealing Temp		Primer Sequence
NT	Neurotensin	NM_024435.2	61°C	Forward	5′-GTG TGG ACC TGC TTG TCA GA-3′
				Reverse	5′-TCA TGC ATG TCT CCT GCT TC-3′
Ntsr1	Neurotensin receptor 1	NM_018766.2	57°C	Forward	5′-TCT GAT GTT GGA CTT GGG TTC-3′
				Reverse	5′-AGT GCT ATG GTA TCT GCT GG-3′
Ntsr2	Neurotensin receptor 2	NM_008747.2	57°C	Forward	5′-TCT CTC AGT TCC CTG TGT GG-3′
				Reverse	5′-AGC AGC CAT TGT TTG TTC TC-3′
Sort1	Sortilin receptor 1/Neurotensin receptor 3	NM_019972.2	57°C	Forward	5′-TTC CCA GAC TAT CCT CAC CC-3′
				Reverse	5′-TAT TGA CCA CAC ACG GCA TC-3′
Ppia	Peptidylprolyl isomerase A	NM_008907.1	58°C	Forward	5′-TGC TGG ACC AAA CAC AAA CG-3′
				Reverse	5′-GCC TTC TTT CAC CTT CCC AAA-3′
Sdha	Succinate dehydrogenase complex, subunit A	NM_023281.1	58°C	Forward	5′-CCG CTC CTA CTG ATG AAA CC-3′
				Reverse	5′-GCG CAA CTC AAT CCC TTA C-3′
Ywhah	Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta	NM_011738.2	57°C	Forward	5′-GAC AAGA GCT GAA TGA ACC AC-3′
				Reverse	5′-TAA CCC TCC AAG AAG ATC GC-3′
Ywhaz	Tyrosine 3-monooxygenase/tryptophan 5 monooxygenase active protein, zeta polypeptide	NM_001253805.1	58°C	Forward	5′-TCC TTA TTC CCT CTT GGC AG-3′
				Reverse	5′-ATG GAA GCT ACA TTA GCG GTT T-3′