Figure 5. A. Heat map of antibody reactivity to FVO Block 2 serotype peptides over the course of immunization. MSP-1 Block 2 specific peptide ELISA is as described in Figure 4 and in Materials and Methods. Reactivities of sera from immunized Aotus are shown as blue rectangles for each peptide tested, with darker colored bars indicating higher ELISA reactivity as shown in the figure key. Columns represent serum reactivity for each time point, and each panel shows reactivity for all pre-challenge samples from each animal. B. Amino acid sequence of the FVO MSP-1 Block 2 antigen. MSP-1 Block 2 flanking sequences are shown in red and internal repeat sequences in blue, matching the peptide sequences shown in Panel A.
