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DO11.10 Tregs induced by OVA injection. Flow cytometry analysis of DO11.10 Tregs (KJ1-26+) in the spleen was conducted after single i.v. injection of OVA. BALB/c mice were adoptively transferred with 1.5 million DO11.10 Tregs (day 0), followed by i.v. injection of 5 μg OVA (day 1). Spleens of transferred mice were harvested on day 4 and day 9 and analysed by flow cytometry.
