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. 2014 Jan 8;9(1):e83808. doi: 10.1371/journal.pone.0083808

Figure 1. Photographic illustration of the steps of the HE-LAMP-BART.

Figure 1

A. Place fecal swab sample into the HE column, mix, seal and twist off the bottom tab of the column; B. Place the column in a collection tube on a hot block at 100°C and incubate for 10 minutes; C. Remove the collection tube and dispose of the column; D&E. Pipette 20 µl of eluate from the collection tube and use this to reconstitute LAMP-BART freeze dried reagent; F&G. Place reconstituted reagent on PDQ and start run; H. Positive results are called in real time. The person in the photograph has given written informed consent, as outlined in the PLOS consent form, to publication of their photograph.