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. 2014 Jan 8;9(1):e84309. doi: 10.1371/journal.pone.0084309

Figure 6. Mixed co-culture.

Figure 6

Survival percentage evaluation of pancreatic islets indirectly co-cultured with MSCs. Calcein-positive cells were counted and the survival percentage was calculated up to 4 weeks of culture. Results are expressed as mean ± SD of three independent experiments. *P<0.05 ** Mixed co-cultures vs Islets, P<0.001 Mixed co-cultures vs Islets and Mixed co-cultures vs Islets+MSCs. Insulin and Pdx1 positive cells in mixed co-cultures made up of pancreatic islets coated with MSCs (within the Transwell, upper panel), and MSCs adhering to the cover glass (lower panel). In green, calcein positive pancreatic islets. In red, MSCs stained with DiI. In blue, insulin or Pdx1 positive cells. Bar 30 µm.