Figure 8. Summary of experiments involving in vitro inactivation of Rsk2 and its consequences on target gene expression, as observed after microinjection in E14.5 mandibular molar explants of a Rsk2 shRNA, followed by qRT-PCR analysis 24 h after injection.

A: Rsk2 was down regulated 4.01 fold after electroporation. B: Eaf2 was significantly down-regulated, and C: Rdh1 was up-regulated after electroporation. Histograms show expression levels in molars electroporated with the random shRNA (blue) and microinjected-electroporated explants with the Rsk2 shRNA (red), with values normalized with respect to Gapdh expression. Data (mean ±SEM) were analyzed with Student t-test; ***p<0.001; **p<0.01; *p<0.05.