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. 2014 Jan 8;9(1):e84663. doi: 10.1371/journal.pone.0084663

Figure 7. 4-PBA inhibits tunicamycin (TM)-induced GRP78 expression in the medulla.

Figure 7

Mice were treated with saline (VEH), TM for 3 days, or pre-treated with 4-PBA for 7 days followed by 3 days of TM and 4-PBA co-treatment. Kidney sections from mice were immunofluorescently stained for GRP78 (arrows) (A). The area of GRP78 staining was determined through wavelength specific thresholding. Results demonstrated that TM treatment led to upregulation of GRP78 expression, mainly in the medullary region of the kidney; this was inhibited by co-treatment with 4-PBA (B). Comparatively less GRP78 staining was found in the cortex of the kidney (C). N = 9. *, P<0.05 vs VEH; #, P<0.05 vs TM.