Figure 2. The antiviral effect of Mmp3 against DENV.

A-B) RNAi efficiency for Mmp3 as shown in qRT-PCR (A) and Western blot (B). C-D) DENV burdens in RAW264.7 cells after RNAi silencing. C) The viral burdens were analyzed by measuring the virus E gene copy using qRT-PCR, and normalized to mouse beta actin gene. D) The titer of infectious DENV in cell supernatants 24 hrs post infection, as measured by TCID₅₀ assay. E) The viral burdens in Mmp3 overexpressed cells were decreased comparing with that in control cells. Results are expressed as the mean + the SEM. * p<0.05 and ** p<0.01(t-test). Representative results from at least 3 independent experiments.