Figure 3. Cytokine expression in DENV infected RAW264.7 cells after gene silencing.

A–F) mRNA levels of selective cytokines/chemokines (A: Ifnβ1, B: Il 6, C:Cxcl1,D:Cxcl2,E:Ccl5,F:Ccr5) were measured by qRT-PCR and normalized to mouse beta-actin gene. G–H) Protein level of TNFα and IL6 in cell supernatants. Results are expressed as the mean + the SEM. * p<0.05 and ** p<0.01. (t-test) Representative results from at least 3 independent experiments.