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. 2014 Jan 8;9(1):e84748. doi: 10.1371/journal.pone.0084748

Figure 4. MMP3 moves from cell cytoplasm into nucleus upon DENV infection and influence NFκB activity.

Figure 4

A) MMP3 moves from cell cytoplasm into nucleus upon DENV infection. B) The cytoplasmic and nuclear distribution of overexpressed flag-MMP3 in DENV infected or uninfected cells as determined by Western Blot. C)NFκB transcriptional activity is impaired upon MMP3 silencing in DENV infected 293T,A549 and RAW264.7 cells, while AP1 transcriptional activities were not influenced. D and E) Overexpression of MMP3 activates NFκB activity in DENV infected RAW264.7 (D) and 293T(E) cells. MMP3 activated NFκB in a dose dependent manner in DENV infected 293T cells(E) (MOI = 1.0, 24 hrs post infection). (The reporter activities were normalized by internal control (pRL-TK Renilla luciferase value). The mean value of activities from DENV infected control cells were set to 1.0) Results are expressed as the mean + the SEM. * p<0.05 and ** p<0.01 (t-test). Representative results from at least 3 independent experiments.