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. 2014 Jan 8;9(1):e84891. doi: 10.1371/journal.pone.0084891

Figure 4. TIFY8 is not induced by JA and is repressed by Pst DC3000 infection.

Figure 4

A–B, RT-PCR analysis of JAZ10 (A) TIFY8 (B) expression after JA treatment. Arabidopsis wild-type seeds were germinated on MS media and, after 8 days, transferred to liquid MS media supplied with different concentrations of JA or COR or equivalent amounts of DMSO (mock treatment). Transcript levels were studied 24 h after treatment. Error bars represent ±SE of four biological replicates. C–D, JAZ10 and TIFY8 expression after infection with Pst DC3000. Transcript levels were studied in 5-week-old Arabidopsis rosette leaves prior to or 24 h after inoculation with Pst DC3000. Error bars represent ±SE of three biological replicates. UBC (AT5G25760) was used as internal control and expression values were normalized to those of the wild-type after mock treatment. (NS, p>0.05; ***, p<0.001, t-test).