Table 1. Overview of prey proteins identified through TAP using TIFY8 as bait.
AGI | Protein | MALDI TOF/TOF mock | Orbitrap Mock | Orbitrap 50 µM JA |
TIFY proteins | ||||
AT4G32570 | TIFY8 | 2 | 2 | 2 |
AT4G14720 | PPD2 | 2 | 2 | 2 |
Repressor proteins | ||||
AT4G28910 | NINJA | 2 | 2 | 2 |
AT1G15750 | TPL | 2 | ||
Other | ||||
AT4G32295 | Unknown | 2 | 2 | 2 |
AT3G24150 | Unknown | 2 | 2 | |
AT3G11540 | SPY | 2 | 2 | |
AT1G51690 | ATB alpha | 2 | 2 | |
AT5G48160 | OBE2 | 2 | 2 | |
AT3G63500 | TTA2 | 2 | 2 | |
AT3G08530/AT3G11130 | Clathrin, heavy chain | 1 | 2 | |
AT4G23460/AT4G11380 | Adaptin family protein | 1 | 1 | |
AT3G25800 | PP2A-4 | 2 | ||
AT2G42500 | PP2A-3 | 2 | ||
AT5G06600 | UBP12 | 2 |
Proteins were identified using peptide-based homology analysis of MS data. Background proteins identified in control experiments were withdrawn. Number indicates the times the prey was identified in 2 experiments with each bait protein. Abbreviations: AGI, Arabidopsis Genome Identifier; PPD2, PEAPOD2; NINJA, NOVEL INTERACTOR OF JAZ; TPL, TOPLESS; SPY, SPINDLY; TTA2, TITANIA2; PP2A, PROTEIN PHOSPHATASE2A; UBP12, UBIQUITIN-SPECIFIC PROTEASE. Detailed MS data can be found in Table S1 and Dataset S1.