Figure 1. Generation of MUC1 SP-specific antibodies.

The specificity and defined minimal epitope of the generated anti-MUC1 SP polyclonal antibodies (A–C) and mAbs SPmAb-2.1 and SPmAb-6 (D–E) were evaluated against the immunizing peptide MUC1-SP-M and its internal epitopes MUC1-SP-S1 to S5, in ELISA assays. MUC1's TRA epitope, MUC1-TRA-L, and the non-MUC1 SP domain BAGE-SP-L, were used in these assays as negative controls.