Table 2. The cellular expression of MUC1 SP domain on MUC1 positive tumor cell-lines and primary naïve cells.
Human tumor Cell-lines Primary cells | Origin | Mouse control | H23 | SPmAb-6 | SPmAb-2.1 | Rabbit control | R23IgG | |
Fluorescence intensity (Geometric mean) | ||||||||
ES-2 | Ovarian Carcinoma | 234 | 232 | 248 | 237 | 251 | 247 | |
OVCAR-3 | Ovarian Carcinoma | 370 | 4456 | 11440 | 1019 | 418 | 10800 | |
MCF7 | Breast Carcinoma | 230 | 1004 | 1091 | 413 | 236 | 4705 | |
MDA-453 | Breast Carcinoma | 182 | 580 | 559 | 290 | 165 | 2443 | |
MDA-231 | Breast Carcinoma | 245 | 966 | 591 | 312 | 215 | 1931 | |
Raji | B-Lymphoblastic Leukemia | 131 | 303 | 307 | 179 | 128 | 519 | |
Ramos | B-Lymphocytic Leukemia | 198 | 558 | 660 | 402 | 111 | 384 | |
U266 | Multiple Myeloma | 485 | 2039 | 661 | 574 | 471 | 1716 | |
RPMI 8226 | Multiple Myeloma | 745 | 2585 | 1190 | 1154 | 738 | 2331 | |
ARH-77 | Plasma cell Leukemia | 176 | 668 | 691 | 439 | 161 | 3966 | |
SK-mel-28 | Melanoma | 117 | 119 | 114 | 112 | 113 | 117 | |
SK-mel-1 | Melanoma-Metastasis | 352 | 325 | 342 | 345 | 350 | 348 | |
Naïve epithelial cells | Mammary epithelial cells (HMEC) | 319 | 338 | 328 | 314 | 380 | 343 | |
Fluorescence intensity (% Positive staining) 1 | ||||||||
T-Lymphocytes (CD3+) | White blood cells | 0 | 0.135 | 0.062 | 0.097 | 0.043 | 0.038 | |
B-Lymphocytes (CD20+) | White blood cells | 0 | 0.031 | 0.003 | 0.009 | 0.003 | 0.41 | |
Myeloid (CD14+) | White blood cells | 0.003 | 0.463 | 0.325 | 0.23 | 0.026 | 0.032 |
1% of positive staining was applies in cell which undergo double staining.