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. 2014 Jan 8;9(1):e85400. doi: 10.1371/journal.pone.0085400

Table 2. The cellular expression of MUC1 SP domain on MUC1 positive tumor cell-lines and primary naïve cells.

Human tumor Cell-lines Primary cells Origin Mouse control H23 SPmAb-6 SPmAb-2.1 Rabbit control R23IgG
Fluorescence intensity (Geometric mean)
ES-2 Ovarian Carcinoma 234 232 248 237 251 247
OVCAR-3 Ovarian Carcinoma 370 4456 11440 1019 418 10800
MCF7 Breast Carcinoma 230 1004 1091 413 236 4705
MDA-453 Breast Carcinoma 182 580 559 290 165 2443
MDA-231 Breast Carcinoma 245 966 591 312 215 1931
Raji B-Lymphoblastic Leukemia 131 303 307 179 128 519
Ramos B-Lymphocytic Leukemia 198 558 660 402 111 384
U266 Multiple Myeloma 485 2039 661 574 471 1716
RPMI 8226 Multiple Myeloma 745 2585 1190 1154 738 2331
ARH-77 Plasma cell Leukemia 176 668 691 439 161 3966
SK-mel-28 Melanoma 117 119 114 112 113 117
SK-mel-1 Melanoma-Metastasis 352 325 342 345 350 348
Naïve epithelial cells Mammary epithelial cells (HMEC) 319 338 328 314 380 343
Fluorescence intensity (% Positive staining) 1
T-Lymphocytes (CD3+) White blood cells 0 0.135 0.062 0.097 0.043 0.038
B-Lymphocytes (CD20+) White blood cells 0 0.031 0.003 0.009 0.003 0.41
Myeloid (CD14+) White blood cells 0.003 0.463 0.325 0.23 0.026 0.032

1% of positive staining was applies in cell which undergo double staining.