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. 2014 Jan 8;9(1):e85402. doi: 10.1371/journal.pone.0085402

Figure 4. NM exposure causes acanthosis in the skin epidermis of SKH-1 and C57BL/6 mice.

Figure 4

Dorsal skin of mice was exposed topically to either 200 µL of acetone or NM (3.2 mg) in 200 µL acetone. After 12, 24, 72 and 120 h of NM exposure, mice were sacrificed and dorsal skin tissue sections (5 µM) were processed, H&E stained and analyzed as detailed under Materials and Methods. Panels A and B are representative H&E stained skin sections (100 or 400× magnification) showing acanthosis from vehicle control as well as NM exposed (72 and 120 h) skin tissue in mice. This NM-related histopathological change was assessed as detailed under materials and methods, and calculated as percent length of mice skin epidermis showing acanthosis (B and D). Data presented are mean ± SEM of 3–5 animals in each group. *, p<0.05 compared to respective vehicle control; VC, vehicle control; NM, nitrogen mustard; e, epidermis; d, dermis; green arrows, acanthosis.