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. 2014 Jan 8;9(1):e85581. doi: 10.1371/journal.pone.0085581

Figure 2. Specificity of ERα deletion in aP2-Cre/ERα females.

Figure 2

(A) RT-PCR analysis of total RNA from inguinal adipose tissue, brown adipose tissue (BAT), liver, uterus, hypothalamus, kidney and muscle from ERαflox/flox and aP2-Cre/ERαflox/flox mice. Arrows indicate the WT ERα transcript (364 bp) and the Cre-deleted ERα transcript lacking exon 3 (176 bp). (B) Relative expression levels of ERα in muscle, kidney, inguinal adipose tissue, visceral adipose tissue, brown adipose tissue, hypothalamus, liver and uterus from ERαflox/flox and aP2-Cre/ERαflox/flox mice. (C) Relative expression levels of Cre in muscle, kidney, inguinal adipose tissue, visceral adipose tissue, brown adipose tissue, hypothalamus, liver and uterus from ERαflox/flox and aP2-Cre/ERαflox/flox mice. Values are given as mean ± SEM; *P<0.05, **P<0.01 and ***P<0.001 vs. control mice.