Fig. 2.

Double immunolabeling with calbindin D28k (A), Tamm-Horsfall protein (B and D), and H⁺-ATPase (C). E-cadherin immunoreactivity was found in the distal convoluted tubule and collecting duct of the pig kidney. In contrast to rat kidney (D), E-cadherin expression was not detected in the thick ascending limb (arrows) of the pig kidney (C). (E) E-cadherin expression in the collecting duct of pig kidney. The level of E-cadherin immunoreactivity (brown) was not detectably different between principal cells (inset, arrowhead) and H+-ATPase-positive intercalated cells (inset, arrow). DCT: distal convoluted tubule, CD: collecting duct, TAL: thick ascending limb. Scale bar = 50 µm.