Figure 3. Niclosamide inhibits breast cancer cell 4T1 and MDA-MB-231 migration and invasion and inhibits FAK-involved pathway.

(A) A total of 5×10⁴ 4T1 cells were seeded in the top chamber of transwell with serum-free medium, and treated with different concentration of niclosamide. After 20 hours, migrated cells were stained, photographed (10×) and quantified. (B) A total of 5×10⁴ 4T1 cells were treated with various concentration of niclosamide and allowed to invade through Matrigel and Transwell membrance, Invaded cell number was stained, photographed (20×) and counted. (C) Niclosamide inhibited MDA-MB-231 migation and invasion. The number of migrated cells and invaded cells was counted, respectively. Data represent means ±SD. (n = 3, in triplicate; *p<0.05; **p<0.01; ***p<0.001). (D) 4T1 cells were treated with different concentration of niclosamide (0–5 µM). After 24 hours, cell lysates were blotted with spectfic antibodies (anti-phospho-STAT3, anti-phospho-FAK, anti-phospho-Src, STAT3, FAK and Src), β-actin was the loading control.