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. 2014 Jan 8;9(1):e85887. doi: 10.1371/journal.pone.0085887

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© 2014 Ye et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Gr1⁺ CD11b⁺ cells were gated and analyzed by FCM for the expression of MDSCs. MDSCs isolated from the tumor tissue of 4T1 tumor-bearing mice were treated with vehicle (A); or treated with niclosamide at 10 mg/kg (B); or treated with niclosamide at 20 mg/kg (C). (D) Statistic results of each group. Treatment of niclosamide significantly reduces the number of MDSCs compared with vehicle group. Values represented means ± SD (n = 6; *p<0.05; **p<0.01).