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Mineralization detection with Tetracycline labeling and two-photon fluorescence imaging. (a) Intra-tubular mineralization in the form of highly fluorescent structures within the dentinal tubules (arrows). (b) Intra-tubular mineralization with globular appearance. A highly fluorescent band is present within the dentin just underneath the cement (c), as well as on the opposing dentin surface of the demineralized disk (d).
