Table 4.
Top scoring 23 representative gene candidates from global overlap in shRNA hairpin screens
| Coverage | Gene | Name | Overlap1 | Biological process |
|---|---|---|---|---|
| GW | KRAS* | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog | 5/9 | Ras protein signal transduction (oncogene) |
| MCM6 | Minichromosome maintenance complex component 6 | 4/9 | Essential for replication inititation | |
| SELS | Selenoprotein S | 4/9 | ER overload response2, degradation of misfolded proteins | |
| SENP5 | Sentrin-specific protease 5 | 4/9 | Essential in SUMO pathway, required for cell division | |
| SNRPC | Small nuclear ribonucleoprotein polypeptide C | 4/9 | Spliceosomal snRNP assembly | |
|
| ||||
| FD | RAD51* | DNA repair protein RAD51 homolog 1 | 18/31 | DNA recombination and repair (oncogene) |
| EIF3E* | Eukaryotic translation initiation factor 3, subunit E | 18/31 | Regulation of translational initiation | |
| CDC5L* | Cell division cycle 5-like | 18/31 | Cell cycle regulator important for G2/M transition | |
| SMG1* | Phosphatidylinositol 3-kinase-related kinase | 18/31 | Nonsense-mediated mRNA decay | |
| CSE1L* | Cellular apoptosis susceptibility protein | 16/31 | Protein transport | |
| MYB | Myeloblastosis viral oncogene homolog | 16/31 | Transcription factor | |
| USP39 | Ubiquitin specific peptidase 39 | 16/31 | mRNA splicing | |
| CDC2L1 | Cyclin-dependent kinase 11B | 15/31 | Cell cycle control | |
| KRAS | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog | 15/31 | Ras protein signal transduction (oncogene) | |
| NR2F1 | Nuclear receptor subfamily 2, group F, member 1 | 15/31 | Transcription initiation | |
| PIK4CA | Phosphatidylinositol 4-kinase | 15/31 | Biosynthesis of phosphatidylinositol 4,5-bisphosphate | |
| TAF10 | TATA box binding protein (TBP)-associated factor | 15/31 | DNA-dependent transcription initiation | |
|
| ||||
| GW & FG | KRAS* | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog | 20/40 | Ras protein signal transduction (oncogene) |
| EIF3E* | Eukaryotic translation initiation factor 3, subunit E | 20/40 | Regulation of translational initiation | |
| CDC5L* | Cell division cycle 5-like | 19/40 | Cell cycle regulator important for G2/M transition | |
| RAD51* | DNA repair protein RAD51 homolog 1 | 19/40 | DNA recombination and repair (oncogene) | |
| SMG1* | Phosphatidylinositol 3-kinase-related kinase | 18/40 | Nonsense-mediated mRNA decay | |
| CSE1L* | Cellular apoptosis susceptibility protein | 18/40 | Protein transport | |
1
Number of gene lists with nominated gene/total number of gene lists in that category,
2
ER; endoplasmic reticulum,
*
Genes repeated in top scoring hits in multiple categories, GW; genome-wide, FD; focused.