Table 6.

Top scoring 32 representative gene candidates from stringent overlap in shRNA hairpin screens

Coverage	Gene	Name	Overlap1	Biological process
GW	EIF5B*	Eukaryotic translation initiation factor 5B	99/105	Translation initiation
	RPS15A*	Ribosomal protein S15a	90/105	Ribosomal (40S) biogenesis, translation
	PSMA1*	Proteasome subunit, α type, 1	83/105	Ubiquitin-dependent peptide cleavage
	RPS8	Ribosomal protein S8	78/105	Ribosomal (40S) biogenesis, translation
	RPS9	Ribosomal protein S9	78/105	Ribosomal (60S) biogenesis, translation
	RPL5*	Ribosomal protein L5	77/105	Ribosomal (60S) biogenesis, rRNA maturation
	EIF3FP3	Eukaryotic translation initiation factor 3	72/105	Pseudogene
	NUP93*	Nucleoporin	72/105	Required for nuclear pore assembly, protein transport
	PSMD4	Proteasome 26S subunit, non-ATPase, 4	64/105	Ubiquitin-dependent peptide cleavage
	RPL23A*	Ribosomal protein L23a	64/105	Ribosomal (60S) biogenesis, translation
	RPS15	Ribosomal protein S15	61/105	Ribosomal (40S) biogenesis, translation
	TUBB	Tubulin β	61/105	Major constituent of microtubules
	NACA	Nascent polypeptide-associated complex α subunit	58/105	Blocks nascent polypeptides interactions with SRP2
	DDX21	DEAD (Asp-Glu-Ala-Asp) box polypeptide 21	56/105	Helicase, foldase (RNA processing)
	DYNC1H1	Dynein cytoplasmic 1 heavy chain 1	56/105	Microtubule-based movement
	SRSF2	Serine/arginine-rich splicing factor 2	56/105	Necessary for the splicing of pre-mRNA
	LSM4	U6 small nuclear RNA associated	55/105	Role in mRNA splicing
	RPL7	Ribosomal protein L7	53/105	Ribosomal (60S) biogenesis, translation
	USP39	Ubiquitin specific peptidase 39	53/105	Likely role in mRNA splicing
	EIF2B3	Eukaryotic translation initiation factor 2B	52/105	Regulation of translation inititation
	RPS25	Ribosomal protein S25	52/105	Ribosomal (40S) biogenesis, translation
FD	RPL5*	Ribosomal protein L5	12/42	Ribosomal (60S) biogenesis, rRNA maturation
	NUP93*	Nucleoporin	11/42	Required for nuclear pore assembly, protein transport
	RPL7*	Ribosomal protein L7	10/42	Ribosomal (60S) biogenesis, translation
	CSNK1E	Casein kinase 1	9/42	DNA replication and repair
	CHEK1	Checkpoint kinase 1	7/42	DNA damage response
	RPA2	Replication protein A2	7/42	DNA replication and repair
GW & FD	EIF5B*	Eukaryotic translation initiation factor 5B	104/147	Translation inititation
	RPS15A*	Ribosomal protein S15a	92/147	Ribosomal (40S) biogenesis, translation
	RPL5*	Ribosomal protein L5	89/147	rRNA maturation, formation of 60S ribosomal subunits
	PSMA1*	Proteasome subunit, α type, 1	88/147	Ubiquitin-dependent peptide cleavage
	NUP93*	Nucleoporin	83/147	Required for nuclear pore assembly, protein transport

¹Number of gene lists with nominated gene/total number of gene lists in that category,

²SRP; signal recognition particle,

^*Genes repeated in top scoring hits in multiple categories, GW; genome-wide, FD; focused.