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. 2014 Jan 8;25(12):1617–1620. doi: 10.1589/jpts.25.1617

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2013© by the Society of Physical Therapy Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 1. — Change of silver-stained 2-DE gels of myoglobin, and expression of myoglobin detected by immunoblotting of rat hind-limb gastrocnemius muscles after seven days of cast immobilization. (A, B-a) Proteins were subjected to IEF on IPG pH 3-10 nonlinear strips and then separated by 12% (w/v) polyacrylamide SDS gels. Statistical analysis was performed on gels from four independent experiments using PDQuest. Arrows show myoglobin protein examined by MALDI-TOF/TOF. (B-b, B-c) Immunoblotting analysis of the cast-immobilized skeletal muscle. *, Significantly different from day 0 of non-cast control, p < 0.05. Con, control; Cast, cast immobilization; pI, isoelectric point; Spinal C, spinal cord; Atro SM, atrophied skeletal muscle; Adrenal G, adrenal gland.