Table 1.
Primer and probes used in real time PCR
| Gene symbol | Gene name | Primer sequence (5’ > 3’) | Probe sequence (5’ > 3’) |
|---|---|---|---|
| SPP1 | osteopontin | F-GCCAGTTGCAGCCTTCTCA R-AAAAGCAAATCACTGCAATTCTCA |
CCAAACGCCGACCAAGGAAAACTCAC |
| COL1A1 | collagen type I alpha1 | F-TAGGGTCTAGACATGTTCAGCTTTGT R-GTGATTGGTGGGATGTCTTCGT |
CCTCTTAGCGGCCACCGCCCT |
| RUNX2 | runt-related transcription factor 2 | F-TCTACCACCCCGCTGTCTTC R-TGGCAGTGTCATCATCTGAAATG |
ACTGGGCTTCCTGCCATCACCGA |
| ALPL | alkaline phosphatase | F-CCGTGGCAACTCTATCTTTGG R-CAGGCCCATTGCCATACAG |
CCATGCTGAGTGACACAGACAAGAAGCC |
| COL3A1 | collagen, type III, alpha 1 | F-CCCACTATTATTTTGGCACAACAG R-AACGGATCCTGAGTCACAGACA |
ATGTTCCCATCTTGGTCAGTCCTATGCG |
| BGLAP | osteocalcin | F-CCCTCCTGCTTGGACACAAA R-CACACTCCTCGCCCTATTGG |
CCTTTGCTGGACTCTGCACCGCTG |
| CD105 | endoglin | F-TCATCACCACAGCGGAAAAA R-GGTAGAGGCCCAGCTGGAA |
TGCACTGCCTCAACATGGACAGCCT |
| FOSL1 | FOS-like antigen 1 | F-CGCGAGCGGAACAAGCT R-GCAGCCCAGATTTCTCATCTTC |
ACTTCCTGCAGGCGGAGACTGACAAAC |
| SP7 | osterix | F-ACTCACACCCGGGAGAAGAA R-GGTGGTCGCTTCGGGTAAA |
TCACCTGCCTGCTCTTGCTCCAAGC |
| RPL13A | ribosomal protein L13 | F-AAAGCGGATGGTGGTTCCT R-GCCCCAGATAGGCAAACTTTC |
CTGCCCTCAAGGTCGTGCGTCTG |