Table 1.
Source, clone and pretreatment for antigen retrieval
| Antibody | Source | Clone | Pretreatment | Dilution | Incubation (min) |
|---|---|---|---|---|---|
| Alpha smooth muscle actin | Dako, Glostrup, Denmark | 1A4 | No treatment | 1:100 | 30 |
| Vimentin | Dako, Glostrup, Denmark | V9 | Microwave Citrate buffer 0.01M pH 7.2 ,95°C, 15 min |
1:100 | 30 |
| Desmin | BioCare, Carmino Diablo, CA, USA |
D33 | Microwave Citrate buffer 0.01M pH 7.2, 95°C, 15 min |
1:100 | 30 |
| Transforming growth factor-beta1 |
Spinge Biosense, Pleasaton, CA, USA |
Polyclonal | Microwave Citrate buffer 0.01M pH 6, 95°C, 10 min |
1:25 | 30 |