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. Author manuscript; available in PMC: 2014 Jan 9.
Published in final edited form as: Int J Dev Neurosci. 2008 Dec 27;27(3):10.1016/j.ijdevneu.2008.12.005. doi: 10.1016/j.ijdevneu.2008.12.005

Table 1.

Primer sequences and annealing temperature used for semiquantative RT-PCR analyses.

cDNA Forward primer (5′–3′) Reverse primer (5′–3′) Annealing temperature (°C) No. PCR cycles Product Reference
TNFα GCCCTAAGGACACCCCTGAGGGAGC TCCAAAGTAGACCTGCCCGGACTCC 58 28 158 bp Yakovlev and Faden (1994).
GAPDH CGGAGTCAACGGATTTGGTCGTAT AGCCTTCTCCATGGTGGTGAAGAC 58 28 306 bp Wong et al. (1994).