Table 1.
Primer sequences and annealing temperature used for semiquantative RT-PCR analyses.
cDNA | Forward primer (5′–3′) | Reverse primer (5′–3′) | Annealing temperature (°C) | No. PCR cycles | Product | Reference |
---|---|---|---|---|---|---|
TNFα | GCCCTAAGGACACCCCTGAGGGAGC | TCCAAAGTAGACCTGCCCGGACTCC | 58 | 28 | 158 bp | Yakovlev and Faden (1994). |
GAPDH | CGGAGTCAACGGATTTGGTCGTAT | AGCCTTCTCCATGGTGGTGAAGAC | 58 | 28 | 306 bp | Wong et al. (1994). |