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. Author manuscript; available in PMC: 2014 Jan 9.
Published in final edited form as: Traffic. 2011 Oct 13;12(12):10.1111/j.1600-0854.2011.01282.x. doi: 10.1111/j.1600-0854.2011.01282.x

Figure 7. BL labeled AREG appears at Ap surface of μ1B-deficient LLC-PK1 cells.

Figure 7

AREG primary antibody was added only to the BL compartment of polarized μ1B-deficient LLC-PK1 cells stably expressing μ1A (LLC-PK1∷μ1A) or μ1B (LLC-PK1∷μ1B) and incubated at 37°C for 30 minutes. Cells were washed and then selectively incubated with Alexa488-conjugated secondary antibody in the BL compartment and Cy5-conjugated secondary antibody in the Ap compartment for 10 minutes at RT. (A) BL primary antibody-bound AREG was detected at the Ap surface in 80% of LLC-PK1∷μ1A cells. Only AREG positive cells were scored that had detectable BL Alexa488 secondary antibody; n=100. (B) A majority of AREG expressing LLC-PK1∷μ1B cells did not have Ap staining (only 35% showing apical Cy5 staining); n=93. The individual channels of the X–Z axis are displayed. Bar, 10 µm.