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. 1974 Aug;71(8):3054–3058. doi: 10.1073/pnas.71.8.3054

A DNA-Protein Complex Involved in Bacteriophage ϕX174 Particle Formation

Peter J Weisbeek 1,*, Robert L Sinsheimer 1
PMCID: PMC388619  PMID: 4528401

Abstract

A ϕX-specific DNA-protein complex has been isolated from ϕX-infected cells. This complex contains infective circular single-stranded DNA, the proteins of the genes F, G, H, and J in the same proportions as in the phage particle, and, in addition, the gene D-protein. The D-protein makes up 12-22% of the protein part of the complex. The sedimentation value of the complex is about 140 S. In vitro, the complex can be converted to the normal 114S phage particle (with a concomitant loss of its D-protein) or to an uninfective 70S particle and a small amount of free single-stranded DNA. The fast sedimenting particle is not associated with membranes.

Keywords: intermediates, assembly, gene D-protein

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benbow R. M., Mayol R. F., Picchi J. C., Sinsheimer R. L. Direction of Translation and Size of Bacteriophage phiX174 Cistrons. J Virol. 1972 Jul;10(1):99–114. doi: 10.1128/jvi.10.1.99-114.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Borrias W. E., van de Pol J. H., van de Vate C., van Arkel G. A. Complementation experiments between conditional lethal mutants of bacteriophage psiX 174. Mol Gen Genet. 1969 Oct 13;105(2):152–163. doi: 10.1007/BF00445685. [DOI] [PubMed] [Google Scholar]
  3. Burgess A. B., Denhardt D. T. Studies on phiX174 proteins. I. Phage-specific proteins synthesized after infection of Escherichia coli. J Mol Biol. 1969 Sep 28;44(3):377–386. doi: 10.1016/0022-2836(69)90367-2. [DOI] [PubMed] [Google Scholar]
  4. GUTHRIE G. D., SINSHEIMER R. L. Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174. Biochim Biophys Acta. 1963 Jun 25;72:290–297. [PubMed] [Google Scholar]
  5. Hutchison C. A., 3rd, Sinsheimer R. L. The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene. J Mol Biol. 1966 Jul;18(3):429–447. doi: 10.1016/s0022-2836(66)80035-9. [DOI] [PubMed] [Google Scholar]
  6. Iwaya M., Denhardt D. T. The mechanism of replication of phi X174 single-stranded DNA. II. The role of viral proteins. J Mol Biol. 1971 Apr 28;57(2):159–175. doi: 10.1016/0022-2836(71)90339-1. [DOI] [PubMed] [Google Scholar]
  7. Knippers R., Strätling W. The DNA replicating capacity of isolated E. coli cell wall-membrane complexes. Nature. 1970 May 23;226(5247):713–717. doi: 10.1038/226713a0. [DOI] [PubMed] [Google Scholar]
  8. Lindqvist B. H., Sinsheimer R. L. Process of infection with bacteriophage phi-X174. XIV. Studies on macromolecular synthesis during infection with a lysis-defective mutant. J Mol Biol. 1967 Aug 28;28(1):87–94. doi: 10.1016/s0022-2836(67)80079-2. [DOI] [PubMed] [Google Scholar]
  9. Mayol R. F., Sinsheimer R. L. Process of infection with bacteriophage phiX174. XXXVI. Measurement of virus-specific proteins during a normal cycle of infection. J Virol. 1970 Sep;6(3):310–319. doi: 10.1128/jvi.6.3.310-319.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Newbold J. E., Sinsheimer R. L. The process of infection with bacteriophage phiX174. XXXII. Early steps in the infection process: attachment, eclipse and DNA penetration. J Mol Biol. 1970 Apr 14;49(1):49–66. doi: 10.1016/0022-2836(70)90375-x. [DOI] [PubMed] [Google Scholar]
  11. Salstrom J. S., Pratt D. Role of coliphage M13 gene 5 in single-stranded DNA production. J Mol Biol. 1971 Nov 14;61(3):489–501. doi: 10.1016/0022-2836(71)90061-1. [DOI] [PubMed] [Google Scholar]
  12. Webster R. E., Cashman J. S. Abortive infection of Escherichia coli with the bacteriophage f1: cytoplasmic membrane proteins and the f1 DNA-gene 5 protein complex. Virology. 1973 Sep;55(1):20–38. doi: 10.1016/s0042-6822(73)81005-0. [DOI] [PubMed] [Google Scholar]
  13. Weisbeek P. J., Van de Pol J. H., Van Arkel G. A. Genetic characterization of the DNA of the bacteriophage phi X174 70 S particle. Virology. 1972 May;48(2):456–462. doi: 10.1016/0042-6822(72)90056-6. [DOI] [PubMed] [Google Scholar]
  14. Zuccarelli A. J., Benbow R. M., Sinsheimer R. L. Deletion mutants of bacteriophage phiX174. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1905–1910. doi: 10.1073/pnas.69.7.1905. [DOI] [PMC free article] [PubMed] [Google Scholar]

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