Table 1.

Primers used for nested PCR amplification of soil fungal and bacterial communities in the rhizosphere of Pinus tabulaeformis.

Primer name	Primer sequence (5′-3′)	Reference
Fungi
ITS1-F	CTTGGTCATTTAGAGGAAGTAA	Gardes and Bruns [25]
ITS4	TCCTCCGCTTATTGATATGC	White et al. [26]
Clamp	CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGG	Gardes and Bruns [25]
ITS1-F-GC	Clamp-CTTGGTCATTTAGAGGAAGTAA	Gardes and Bruns [25]
ITS2	GCTGCGTTCTTCATCGATGC	White et al. [26]
Bacteria
fD1	AGAGTTTGATCCTGGCTCAG	Weisburg et al. [27]
rP1	ACGGTTACCTTGTTACGACTT	Weisburg et al. [27]
341f	CCTACGGGAGGCAGCAG	Muyzer et al. [28]
341f-GC	Clamp-CCTACGGGAGGCAGCAG	Muyzer et al. [28]
534r	ATTACCGCGGCTGCTGG	Muyzer et al. [28]