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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Microbes Infect. 2013 Oct 18;15(0):10.1016/j.micinf.2013.09.011. doi: 10.1016/j.micinf.2013.09.011

Figure 4. TRP120 directly binds host genomic DNA at a specific motif and modulates host gene expression.

Figure 4

A, EMSA demonstrates recombinant TRP-TR directly binds biotin-labeled mammalian genomic DNA (purified from THP-1 human monocytes). Incubation with epitope-specific TRP120 antibody results in a supershift of the TRP120-TR-DNA complex. B, GC-rich consensus binding motif identified from TRP120 ChIPSeq peak analysis using WebLogo. C, Gene ontology biological process classification of host genes identified from TRP120-TR ChIP analysis. D, Induction of expression for select TRP120 target genes is observed in THP-1 cells 72 h post E. chaffeensis infection (relative to uninfected THP-1 cells) or recombinant TRP120 transfection (relative to thioredoxin control protein), as determined by qPCR analysis. Figure is a modified reprint from [48].