Figure 4. BMDM and BMDC from Optn^470T mice have unimpaired NF-κB responses.

BMDM from WT and Optn^470T mice were stimulated with TNF for the indicated times and degradation of IκBα was detected by immunoblotting (A). BMDM pretreated overnight with 300 U/ml of IFN-β or not were stimulated with TNF as indicated, and lysates were blotted for optineurin and IκBα (B). Cell lysates of TNF-treated BMDM were blotted for P-ERK (C). Cell lysates of LPS-stimulated BMDM were blotted for IκBα (D). A representative example for two to three experiments is shown for all blots, with β-actin staining as a loading control. The indicated cytokines secreted upon LPS stimulation of Optn^470T BMDM (E) and BMDC (F) are depicted as the percent of WT values. The data from for two to three independent experiments are shown as mean ± SEM. Cytokines detected in the sera of LPS-injected WT and Optn^470T BMDM are shown (G). The mean ± SEM from 4 mice from two independent experiments is shown.