Table I. Optn470T mice on the 129 x B6 genetic background die embryonically.
Heterozygous OptnWT/470T F3 mice were intercrossed and the genotype of the offspring was analyzed by PCR from tail DNA of adult mice or mouse embryonic fibroblasts generated from E11.5–E13.5 day old embryos. The expected and observed frequencies from 135 adult mice and 20 embryos are shown.
| WT/Optn470T x WT/Optn470T | WT/WT | WT/Optn470T | Optn470T/Optn470T |
|---|---|---|---|
| expected frequency | 25% | 50% | 25% |
| adult mice (number) | 48 | 83 | 4 |
| observed frequency of adult mice | 35% | 61% | 3% |
| developed embryos (number) | 5 | 14 | 1 |
| observed frequency of developed embryos | 25% | 70% | 5% |