. Author manuscript; available in PMC: 2014 Dec 15.

Published in final edited form as: J Immunol. 2013 Nov 15;191(12):10.4049/jimmunol.1301696. doi: 10.4049/jimmunol.1301696

Table II. Optn^470T mice further backcrossed to B6 background were born at the expected Mendelian ratios.

Heterozygous Optn^WT/470T mice backcrossed onto the B6 background for five generations (F5) were intercrossed and the genotype of the offspring was analyzed by PCR of tail DNA. The expected and observed frequencies of 67 adult mice are shown.

WT/Optn^470T x WT/Optn^470T	WT/WT	WT/Optn^470T	Optn^470T/Optn^470T
expected frequency	25%	50%	25%
adult mice (number)	14	38	15
observed frequency of adult mice	21%	57%	22%

Table II. Optn470T mice further backcrossed to B6 background were born at the expected Mendelian ratios.

Table II. Optn^470T mice further backcrossed to B6 background were born at the expected Mendelian ratios.