Figure 3.

Leech neurons were grown on T2, T4 and FLAT substrates for 4 days. (a) Tubulin cytoskeleton directionality analysis. The FFT tubulin fluorescence signal aligned to the GR (within 16°) is reported for different GRs. (b) Leech neurites were traced and the total length of the neurite network aligned to the GR (alignment angle less than or equal to 15°) is reported as the percentage over the total neurite network length for the different substrates. (c) Mean alignment angle of neurite segments for the different substrates. (a,b) *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA, Tukey's test; (c) #p < 0.001, one-way ANOVA, Kruskal–Wallis test. Data are reported as mean ± s.d.